Optimal amplification conditions for D16S3399 polymorphic STS axin-1 gene marker
نویسندگان
چکیده
We investigated D16S3399 marker and affirmed it as a highly polymorphic marker useful for the analysis of the human axin-1 gene. Axin-1 acts as a tumor suppressor gene and its protein is an inhibitor of the Wnt signaling pathway. We report on heterozygosity status, alleles frequency observed in a preliminary group of Croatian subjects and the optimal amplification conditions for D16S3399 marker. The amplified CA repeat was confirmed by direct sequencing.
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